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KMID : 0545120030130030373
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Journal of Microbiology and Biotechnology
2003 Volume.13 No. 3 p.373 ~ p.377
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Ion-Sensitive Field Effect Transistor-Based Multienzyme Sensor for Alternative Detection of Mercury Ions, Cyanide, and Pesticide
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Vyacheslav V
Kim NS
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Abstract
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Various groups of industrial and agricultural pollutants (heavy metal ions cyanides and pesticides) can be detected by enzymes. Since heavy metal ions inhibit urease cyanides inhibit peroxidase organophosphorus and carbamate pesticides inhibit butyrylcholinesterase these enzymes were co-immobilized into a bovine serum albumin gel on the surface of an ionsensitive field effect transistor to create a bioprobe that is sensitive to the compounds mentioned above. The sensitivity of the present sensor towards KCN corresponded to 1 mM with 1 min of incubation time. The detection limits for Hg(II) ions and the pesticide carbofuran were 0.1 and 0.5 mM respectively when a 10 min sensor incubation time in contaminated samples was chosen. The total time for determining the concentrations of all species mentioned did not exceed 20 min.
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